Skip to main content
Addgene

pgRNA-R-gfp
(Plasmid #220927)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pgRNA_AT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    superfolder GFP
  • Species
    Synthetic
  • Insert Size (bp)
    797
  • GenBank ID
    AJW68299.1
  • Promoter J23119
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Amp-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgRNA-R-gfp was a gift from Andrea Puhar (Addgene plasmid # 220927 ; http://n2t.net/addgene:220927 ; RRID:Addgene_220927)

  • For your References section:

    CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing. Sharma A, Omer Aden R, Puhar A, Cisneros DA. mSystems. 2022 Dec 21:e0104522. doi: 10.1128/msystems.01045-22. 10.1128/msystems.01045-22 PubMed 36541764
Related items: