plenti hSyn GAPDH
(Plasmid
#220918)
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PurposeNeuronal specific expression of GAPDH
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-hSyn
- Backbone size w/o insert (bp) 8462
- Total vector size (bp) 9470
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAPDH
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Alt nameglyceraldehyde-3-phosphate dehydrogenase
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SpeciesH. sapiens (human)
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Insert Size (bp)1008
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Entrez GeneGAPDH (a.k.a. G3PD, GAPD, HEL-S-162eP)
- Promoter hSyn
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer hSyn-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti hSyn GAPDH was a gift from Timothy Ryan (Addgene plasmid # 220918 ; http://n2t.net/addgene:220918 ; RRID:Addgene_220918)