plenti hSyn AldoA
(Plasmid
#220916)
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PurposeNeuronal specific expression of AldoA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-hSyn
- Backbone size w/o insert (bp) 8432
- Total vector size (bp) 9527
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAldoA
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Alt namealdolase, fructose-bisphosphate A
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SpeciesH. sapiens (human)
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Insert Size (bp)1095
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Entrez GeneALDOA (a.k.a. ALDA, GSD12, HEL-S-87p)
- Promoter hSyn
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer hSyn-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A P72H mutation was found in AldoA. Please visit https://doi.org/10.1101/2023.10.10.561760 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti hSyn AldoA was a gift from Timothy Ryan (Addgene plasmid # 220916 ; http://n2t.net/addgene:220916 ; RRID:Addgene_220916) -
For your References section:
Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits. Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, Kaplitt MG, De Camilli P, Ryan TA. Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. Epub 2024 Aug 21. 10.1126/sciadv.adn6016 PubMed 39167658