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Addgene

pJMP8835
(Plasmid #220902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    R6Kgamma
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Growth instructions
    Plasmid contains both Ampicillin (backbone) and Kanamycin (transposon) resistance genes. Either antibiotic can be used for plasmid growth and propagation but for selection of downstream transposition events Kanamycin must be used.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dCas9-novo
  • gRNA/shRNA sequence
    PD-sgRNA (mscar targeting)
  • Species
    S. pyogenes
  • Mutation
    D10A, H840A
  • Tag / Fusion Protein
    • 3xMyc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mobile-CRISPRi system optimized for alphaproteobacteria; needs to be grown in E. coli BW25141 (pir+ strain with an anti-CRISPR protein on the chromosome)

Please visit https://pubmed.ncbi.nlm.nih.gov/37662258/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMP8835 was a gift from Jason Peters (Addgene plasmid # 220902 ; http://n2t.net/addgene:220902 ; RRID:Addgene_220902)

  • For your References section:

    Tools for genetic engineering and gene expression control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides. Hall AN, Hall BW, Kinney KJ, Olsen GG, Banta AB, Noguera DR, Donohue TJ, Peters JM. Appl Environ Microbiol. 2024 Sep 26:e0034824. doi: 10.1128/aem.00348-24. 10.1128/aem.00348-24 PubMed 39324814
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