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Addgene

pJMP8045
(Plasmid #220900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIND4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tnsABCD
  • Species
    E. coli

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Needs to be grown in E. coli BW25141 (pir+ strain with an anti-CRISPR protein on the chromosome)

Please visit https://pubmed.ncbi.nlm.nih.gov/37662258/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMP8045 was a gift from Jason Peters (Addgene plasmid # 220900 ; http://n2t.net/addgene:220900 ; RRID:Addgene_220900)

  • For your References section:

    Tools for genetic engineering and gene expression control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides. Hall AN, Hall BW, Kinney KJ, Olsen GG, Banta AB, Noguera DR, Donohue TJ, Peters JM. Appl Environ Microbiol. 2024 Sep 26:e0034824. doi: 10.1128/aem.00348-24. 10.1128/aem.00348-24 PubMed 39324814
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