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Addgene

mFGF8b
(Plasmid #22090)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGF8
  • Alt name
    FGF8 splice variant 1(B)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    880
  • Entrez Gene
    Fgf8 (a.k.a. Aigf, Fgf-8, Fgf6c, HBGF-8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse FGF8 cDNA splice variant 1(B) clone was isolated from an E7.5 cDNA library. The insert contains the entire coding region and 3' UTR. The SmaI site is in the FGF8 5'UTR 16bp upstream of the translation start site. The 3' XhoI site is from the vector MCS. The FGF8 variant 1(B) sequence is ~30bp longer than the variant 4(A) sequence.

For antisense probe linearize with BamHI and use T7 Polymerase. The transcript is ~875bp.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mFGF8b was a gift from Gail Martin (Addgene plasmid # 22090 ; http://n2t.net/addgene:22090 ; RRID:Addgene_22090)

  • For your References section:

    The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Crossley PH, Martin GR. Development. 1995 Feb . 121(2):439-51. PubMed 7768185
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