pCMV-haA3A-CBE-VA
(Plasmid
#220893)
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PurposeExpress haA3A-CBE-VA (with SpCas9) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV with a BR322 origin
- Total vector size (bp) 8958
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehaA3A-CBE-VA
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Insert Size (bp)5559
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-haA3A-CBE-VA was a gift from Dali Li (Addgene plasmid # 220893 ; http://n2t.net/addgene:220893 ; RRID:Addgene_220893) -
For your References section:
Engineering APOBEC3A deaminase for highly accurate and efficient base editing. Yang L, Huo Y, Wang M, Zhang D, Zhang T, Wu H, Rao X, Meng H, Yin S, Mei J, Zhang D, Chen X, Lv J, Liu M, Cheng Y, Guan Y, Feng B, Song G, Yi C, Liu M, Zeng F, Wang L, Li D. Nat Chem Biol. 2024 Mar 29. doi: 10.1038/s41589-024-01595-4. 10.1038/s41589-024-01595-4 PubMed 38553609