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Addgene

cFGF8
(Plasmid #22089)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK (-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGF8
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    800
  • GenBank ID
    U41467
  • Entrez Gene
    FGF8 (a.k.a. FGF-8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region.

For antisense probe linearize with EcoRI and use T7 Polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cFGF8 was a gift from Gail Martin (Addgene plasmid # 22089 ; http://n2t.net/addgene:22089 ; RRID:Addgene_22089)

  • For your References section:

    Roles for FGF8 in the induction, initiation, and maintenance of chick limb development. Crossley PH, Minowada G, MacArthur CA, Martin GR. Cell. 1996 Jan 12. 84(1):127-36. 10.1016/S0092-8674(00)80999-X PubMed 8548816
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