8VL7
(Plasmid
#220883)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene Plasmid #26096)
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Vector typeBacterial Expression
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Selectable markerspET28-MHL
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTREX1
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Alt nameTREX1_2-234a
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Alt nameTREX1:PBC021-E11:C244990
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SpeciesH. sapiens (human)
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Insert Size (bp)696
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Entrez GeneTREX1 (a.k.a. AGS1, CRV, DRN3, HERNS, RVCLS)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 8VL7. https://www.rcsb.org/structure/8VL7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
8VL7 was a gift from Cheryl Arrowsmith (Addgene plasmid # 220883 ; http://n2t.net/addgene:220883 ; RRID:Addgene_220883)