avi-eTMPRSS11D S368A
(Plasmid
#220882)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFHMSPN-avi-TEV-LIC
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Vector typeBaculovirus Expresssion
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Selectable markerspFHMSPN-avi-TEV-LIC
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTMPRSS11D
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Alt nameTMPRSS11D:TOC038-C02:C249372
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SpeciesH. sapiens (human)
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Insert Size (bp)1125
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MutationS368A
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Entrez GeneTMPRSS11D (a.k.a. ASP, HAT)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- Honeybee melittinsignal (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
avi-eTMPRSS11D S368A was a gift from Cheryl Arrowsmith (Addgene plasmid # 220882 ; http://n2t.net/addgene:220882 ; RRID:Addgene_220882)
