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Addgene

avi-eTMPRSS11D S368A
(Plasmid #220882)

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Item Catalog # Description Quantity Price (USD)
Plasmid 220882 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFHMSPN-avi-TEV-LIC
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    pFHMSPN-avi-TEV-LIC

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMPRSS11D
  • Alt name
    TMPRSS11D:TOC038-C02:C249372
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1125
  • Mutation
    S368A
  • Entrez Gene
    TMPRSS11D (a.k.a. ASP, HAT)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • Honeybee melittinsignal (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    avi-eTMPRSS11D S368A was a gift from Cheryl Arrowsmith (Addgene plasmid # 220882 ; http://n2t.net/addgene:220882 ; RRID:Addgene_220882)
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