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Addgene

8VIS
(Plasmid #220881)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220881 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFHMSP-LIC-C
  • Backbone manufacturer
    SGC (Addgene Plasmid #26100)
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    pFHMSP-LIC-C

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMPRSS11D
  • Alt name
    dasTMPRSS11D
  • Alt name
    TMPRSS11D:TOC036-C02:C249111
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1125
  • Entrez Gene
    TMPRSS11D (a.k.a. ASP, HAT)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • EFVEHHHHHHHH (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8VIS was a gift from Cheryl Arrowsmith (Addgene plasmid # 220881 ; http://n2t.net/addgene:220881 ; RRID:Addgene_220881)
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