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Addgene

TMPRSS3:72-454_CO
(Plasmid #220880)

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Item Catalog # Description Quantity Price (USD)
Plasmid 220880 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFHMSP-LIC-C
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    pFastBac HTA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMPRSS3
  • Alt name
    TMPRSS3:72-454_CO
  • Alt name
    TMPRSS3:TOC031-D05:C246773
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1218
  • Entrez Gene
    TMPRSS3 (a.k.a. DFNB10, DFNB8, ECHOS1, TADG12)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • melittin signal sequence (N terminal on insert)
    • 8xHis (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMPRSS3:72-454_CO was a gift from Cheryl Arrowsmith (Addgene plasmid # 220880 ; http://n2t.net/addgene:220880 ; RRID:Addgene_220880)
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