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Addgene

8V04
(Plasmid #220879)

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Item Catalog # Description Quantity Price (USD)
Plasmid 220879 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFHMSP-LIC-C
  • Backbone manufacturer
    SGC (Addgene Plasmid #26100)
  • Vector type
    Baculovirus Expresssion
  • Selectable markers
    pFHMSP-LIC-C

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMPRSS2
  • Alt name
    etmprss2_148-492_S251D/R252D/Q253D/S254D/R255K, TMPRSS2:JMC152-E08:C246676
  • Alt name
    8V1F
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1035
  • Mutation
    S251D/R252D/Q253D/S254D/R255K
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • Honeybee melittin signal (N terminal on backbone)
    • EFVEHHHHHHHH (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8V04 was a gift from Cheryl Arrowsmith (Addgene plasmid # 220879 ; http://n2t.net/addgene:220879 ; RRID:Addgene_220879)
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