SCML2_3
(Plasmid
#220878)
-
PurposeBaculovirus expression for structure determination; may not be full ORF
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET28-MHL
-
Backbone manufacturerSGC (Addgene Plasmid #26096)
-
Vector typeBacterial Expression
-
Selectable markerspET28-MHL
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameSCML2
-
Alt nameSCML2:APC052-E07:C30254
-
SpeciesH. sapiens (human)
-
Insert Size (bp)645
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SCML2_3 was a gift from Cheryl Arrowsmith (Addgene plasmid # 220878 ; http://n2t.net/addgene:220878 ; RRID:Addgene_220878) -
For your References section:
Histone recognition by human malignant brain tumor domains. Nady N, Krichevsky L, Zhong N, Duan S, Tempel W, Amaya MF, Ravichandran M, Arrowsmith CH. J Mol Biol. 2012 Nov 9;423(5):702-18. doi: 10.1016/j.jmb.2012.08.022. Epub 2012 Sep 4. 10.1016/j.jmb.2012.08.022 PubMed 22954662
