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Addgene

pLYS6-METTL17-LYRMut-FLAG
(Plasmid #220877)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLYS6
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    METTL17
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1471
  • Mutation
    Changed the LYP domain to AAA
  • Entrez Gene
    METTL17 (a.k.a. METT11D1)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLYS6-METTL17-LYRMut-FLAG was a gift from Vamsi Mootha (Addgene plasmid # 220877 ; http://n2t.net/addgene:220877 ; RRID:Addgene_220877)

  • For your References section:

    METTL17 is an Fe-S cluster checkpoint for mitochondrial translation. Ast T, Itoh Y, Sadre S, McCoy JG, Namkoong G, Wengrod JC, Chicherin I, Joshi PR, Kamenski P, Suess DLM, Amunts A, Mootha VK. Mol Cell. 2024 Jan 18;84(2):359-374.e8. doi: 10.1016/j.molcel.2023.12.016. Epub 2024 Jan 9. 10.1016/j.molcel.2023.12.016 PubMed 38199006
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