lentiMutB3GALT5catdead-blast
(Plasmid
#220869)
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Purposelentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonelentiCas9-blast
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Backbone manufacturerAddgene 52962
- Backbone size w/o insert (bp) 8669
- Total vector size (bp) 9719
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-1,3-galactosyltransferase 5
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Alt nameB3GALT5
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SpeciesH. sapiens (human)
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Insert Size (bp)930
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Mutationencodes D156A and D243A mutations; also, nucleotide change in PAM targeting sequence nt 515 g->a
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GenBank IDNM_033173
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Entrez GeneB3GALT5 (a.k.a. B3GalT-V, B3GalTx, B3T5, GLCT5, beta-1,3-GalTase 5, beta-3-Gx-T5, beta3Gal-T5)
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Tag
/ Fusion Protein
- myc, FLAG (N terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOrigene CAT#: RC211183
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.08.02.551727 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lentiMutB3GALT5catdead-blast was a gift from Jennifer Kohler (Addgene plasmid # 220869 ; http://n2t.net/addgene:220869 ; RRID:Addgene_220869)