pDisplay-UDP1.0-IRES-mCherry-CAAX
(Plasmid
#220850)
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PurposeCMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDisplay
- Backbone size w/o insert (bp) 6537
- Total vector size (bp) 8376
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUDP1.0
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Alt nameGRAB_UDP1.0
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATGGGCGGTAGGCGTGTA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An insertion of D and L aa's between D157 and Q158 in NeoR/KanR was observed. This may affect its function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDisplay-UDP1.0-IRES-mCherry-CAAX was a gift from Yulong Li (Addgene plasmid # 220850 ; http://n2t.net/addgene:220850 ; RRID:Addgene_220850) -
For your References section:
Microglial P2Y(6) calcium signaling promotes phagocytosis and shapes neuroimmune responses in epileptogenesis. Umpierre AD, Li B, Ayasoufi K, Simon WL, Zhao S, Xie M, Thyen G, Hur B, Zheng J, Liang Y, Bosco DB, Maynes MA, Wu Z, Yu X, Sung J, Johnson AJ, Li Y, Wu LJ. Neuron. 2024 Apr 10:S0896-6273(24)00195-8. doi: 10.1016/j.neuron.2024.03.017. 10.1016/j.neuron.2024.03.017 PubMed 38614103