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Addgene

mFGF4
(Plasmid #22085)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGF4
  • Alt name
    Fgfk (clone K2)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    620
  • Entrez Gene
    Fgf4 (a.k.a. Fgf-4, Fgf7a, Fgfk, HBGF-4, Hst1, Hstf-1, KS3, hst, hst-1, kFGF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length coding region of K-FGF, PCR clone. The insert can be cut out with EcoRI and BamHI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mFGF4 was a gift from Gail Martin (Addgene plasmid # 22085 ; http://n2t.net/addgene:22085 ; RRID:Addgene_22085)

  • For your References section:

    Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Hebert JM, Basilico C, Goldfarb M, Haub O, Martin GR. Dev Biol. 1990 Apr . 138(2):454-63. 10.1016/0012-1606(90)90211-Z PubMed 2318343
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