pAAV-hSyn-UDP1.0-WPRE
(Plasmid
#220849)
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PurposeAAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-hSyn
- Backbone size w/o insert (bp) 4495
- Total vector size (bp) 6334
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUDP1.0
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Alt nameGRAB_UDP1.0
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SpeciesSynthetic
- Promoter human Synapsin (hSyn)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCCCATTCCCCAAATTGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-UDP1.0-WPRE was a gift from Yulong Li (Addgene plasmid # 220849 ; http://n2t.net/addgene:220849 ; RRID:Addgene_220849) -
For your References section:
Microglial P2Y(6) calcium signaling promotes phagocytosis and shapes neuroimmune responses in epileptogenesis. Umpierre AD, Li B, Ayasoufi K, Simon WL, Zhao S, Xie M, Thyen G, Hur B, Zheng J, Liang Y, Bosco DB, Maynes MA, Wu Z, Yu X, Sung J, Johnson AJ, Li Y, Wu LJ. Neuron. 2024 Apr 10:S0896-6273(24)00195-8. doi: 10.1016/j.neuron.2024.03.017. 10.1016/j.neuron.2024.03.017 PubMed 38614103