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Addgene

pENN239_CRISPRcharm_Kv1 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Ladder and a digestion of the plasmid pENN239_CRISPRcharm_Kv1 with the following enzymes: AgeI-HF, SacI-HF

Expected bands:
For a AgeI-HFSacI-HF double digest, we expect to see two bands at 7.7 + 3.4 kb.

Results:
We observe two bands (~3.4kb, ~7.8kb).