pCM98
(Plasmid
#220831)
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PurposeEryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM98 was a gift from Matthew Bennett (Addgene plasmid # 220831 ; http://n2t.net/addgene:220831 ; RRID:Addgene_220831) -
For your References section:
Macrolide Biosensor Optimization through Cellular Substrate Sequestration. Miller CA, Ho JM, Parks SE, Bennett MR. ACS Synth Biol. 2021 Feb 19;10(2):258-264. doi: 10.1021/acssynbio.0c00572. Epub 2021 Feb 8. 10.1021/acssynbio.0c00572 PubMed 33555859