pT7-NSP1delta103CSA11-GFP
(Plasmid
#220813)
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PurposeExpress the Rotavirus SA11 delta103C NSP1 protein fused with the sfGFP (Pedelacq et al., 2006)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT7
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRotavirus SA11 NSP1delta103C-GFP
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SpeciesRotavirus SA11
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MutationLacks C-terminal 103 aa
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-NSP1delta103CSA11-GFP was a gift from Alex Borodavka (Addgene plasmid # 220813 ; http://n2t.net/addgene:220813 ; RRID:Addgene_220813)