pBS119d10L
(Plasmid
#22081)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II KS
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Backbone manufacturerStrategene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepBS119d10L
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Alt namepfli-short
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)7000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To view partial vector sequence from the depositing lab, please click on the Reviews link.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS119d10L was a gift from Brant Weinstein (Addgene plasmid # 22081 ; http://n2t.net/addgene:22081 ; RRID:Addgene_22081) -
For your References section:
In vivo imaging of embryonic vascular development using transgenic zebrafish. Lawson ND, Weinstein BM. Dev Biol. 2002 Aug 15. 248(2):307-18. 10.1006/dbio.2002.0711 PubMed 12167406