pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP
(Plasmid
#220797)
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PurposeshRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-H1-shRNA-hsynapsin-EGFP-WPRE
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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gRNA/shRNA sequenceSRGAP2B and SRGAP2C
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.03.01.530630 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP was a gift from Pierre Vanderhaeghen (Addgene plasmid # 220797 ; http://n2t.net/addgene:220797 ; RRID:Addgene_220797) -
For your References section:
Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition. Libe-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, Gaspariunaite V, Peze-Heidsieck E, Remans D, Charrier C, Theys T, Polleux F, Vanderhaeghen P. Neuron. 2024 Oct 9:S0896-6273(24)00645-7. doi: 10.1016/j.neuron.2024.08.021. 10.1016/j.neuron.2024.08.021 PubMed 39406239