pAceBAC2-MLG-HMF-LST2
(Plasmid
#220787)
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PurposeExpresses N-terminal His10-Myc-FLAG-LST2 in insect cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAceBAC2-HMF
- Backbone size w/o insert (bp) 2926
- Total vector size (bp) 5587
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLST2
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Alt nameZFYVE28
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SpeciesH. sapiens (human)
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Insert Size (bp)2661
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Entrez GeneZFYVE28 (a.k.a. LST2, LYST2, lst-2)
- Promoter p10
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Tag
/ Fusion Protein
- 10xHis-Myc-FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attB1
- 3′ sequencing primer attB2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAceBAC2-MLG-HMF-LST2 was a gift from Timm Maier (Addgene plasmid # 220787 ; http://n2t.net/addgene:220787 ; RRID:Addgene_220787) -
For your References section:
mTORC1 phosphorylates and stabilizes LST2 to negatively regulate EGFR. Battaglioni S, Craigie L, Filippini S, Maier T, Hall MN. PNAS 2024