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Addgene

pCMV-MLG-HMF-LST2
(Plasmid #220786)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220786 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV-MLG-HMF
  • Backbone size w/o insert (bp) 5575
  • Total vector size (bp) 8236
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LST2
  • Alt name
    ZFYVE28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8236
  • Entrez Gene
    ZFYVE28 (a.k.a. LST2, LYST2, lst-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 10xHis-Myc-FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-MLG-HMF-LST2 was a gift from Timm Maier (Addgene plasmid # 220786 ; http://n2t.net/addgene:220786 ; RRID:Addgene_220786)
  • For your References section:

    mTORC1 phosphorylates and stabilizes LST2 to negatively regulate EGFR. Battaglioni S, Craigie L, Filippini S, Maier T, Hall MN. PNAS 2024