pGLO-Blue
(Plasmid #220782)

• Purpose: Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)
• Depositing Lab: Angela Leu
• Publication: Bacterial expression plasmids for education and teaching (unpublished)

Backbone

• Vector backbone: pGLO
• Backbone manufacturer: BioRad
• Backbone size w/o insert (bp): 5371
• Total vector size (bp): 6176
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: blue chromoprotein (aeBlue)
• Insert Size (bp): 714
• Tag / Fusion Protein:
  • 8xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: CAC ATG GCA TGG ATG AGC
• 3′ sequencing primer: CTC ATC CGC CAA AAC AGC

Resource Information

• Supplemental Documents:
  • pGLO-Blue.gb
• A portion of this plasmid was derived from a plasmid made by: The backbone plasmid (pGLO) was purchased by Bio-Rad. It is an educational plasmid. For the insert it was used the plasmids of amino labs (https://amino.bio/products/engineer-it-kit)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry