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Addgene

pGLO-Blue
(Plasmid #220782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGLO
  • Backbone manufacturer
    BioRad
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 6176
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    blue chromoprotein (aeBlue)
  • Insert Size (bp)
    714
  • Tag / Fusion Protein
    • 8xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer CAC ATG GCA TGG ATG AGC
  • 3′ sequencing primer CTC ATC CGC CAA AAC AGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The backbone plasmid (pGLO) was purchased by Bio-Rad. It is an educational plasmid. For the insert it was used the plasmids of amino labs (https://amino.bio/products/engineer-it-kit)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGLO-Blue was a gift from Angela Leu (Addgene plasmid # 220782 ; http://n2t.net/addgene:220782 ; RRID:Addgene_220782)
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