Skip to main content
Addgene

BmTyr-Flag
(Plasmid #220779)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-N
  • Modifications to backbone
    EGFP was omitted
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bacillus megaterium Tyrosinase
  • Alt name
    BmTyr
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BmTyr-Flag was a gift from Itaru Hamachi (Addgene plasmid # 220779 ; http://n2t.net/addgene:220779 ; RRID:Addgene_220779)

  • For your References section:

    Tyrosinase-Based Proximity Labeling in Living Cells and In Vivo. Zhu H, Oh JH, Matsuda Y, Mino T, Ishikawa M, Nakamura H, Tsujikawa M, Nonaka H, Hamachi I. J Am Chem Soc. 2024 Mar 20;146(11):7515-7523. doi: 10.1021/jacs.3c13183. Epub 2024 Mar 6. 10.1021/jacs.3c13183 PubMed 38445591
Related items: