pAV-CAG-Sun1-GFP-WPRE-BC10-pA
(Plasmid
#220762)
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PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAV-CAG-Sun1-GFP-WPRE-pA
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Backbone manufacturerSamineni Lab (Addgene #220752)
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSun1-GFP-WPRE-BC10
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SpeciesSynthetic
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site HpaI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.04.24.590975 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV-CAG-Sun1-GFP-WPRE-BC10-pA was a gift from Vijay Samineni (Addgene plasmid # 220762 ; http://n2t.net/addgene:220762 ; RRID:Addgene_220762) -
For your References section:
Projection-TAGs enable multiplex projection tracing and multi-modal profiling of projection neurons. Yang L, Liu F, Hahm H, Okuda T, Li X, Zhang Y, Kalyanaraman V, Heitmeier MR, Samineni VK. bioRxiv 2024.04.24.590975 10.1101/2024.04.24.590975