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Addgene

pAD-IRP
(Plasmid #220751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACTII
  • Total vector size (bp) 7800
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Iron Regulatory Protein
  • GenBank ID
    M95815.1
  • Entrez Gene
    ACO1 (a.k.a. FRP)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was used as a positive control in the cited paper. I did not sequence it, but have provided the unpublished information about the Yeast Three Hybrid plasmids for the Wickens web site for further reference.

https://wickenslab.biochem.wisc.edu/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD-IRP was a gift from Marvin Wickens (Addgene plasmid # 220751 ; http://n2t.net/addgene:220751 ; RRID:Addgene_220751)

  • For your References section:

    Analyzing mRNA-protein complexes using a yeast three-hybrid system. Bernstein DS, Buter N, Stumpf C, Wickens M. Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4. 10.1016/S1046-2023(02)00015-4 PubMed 12054889
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