pAD-IRP
(Plasmid
#220751)
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PurposePlasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACTII
- Total vector size (bp) 7800
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIron Regulatory Protein
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GenBank IDM95815.1
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Entrez GeneACO1 (a.k.a. FRP)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ctgcaggtcgactctagaggatcgGAAttCCCCC AtAtCCAACttCCAAtttAAtC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was used as a positive control in the cited paper. I did not sequence it, but have provided the unpublished information about the Yeast Three Hybrid plasmids for the Wickens web site for further reference.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD-IRP was a gift from Marvin Wickens (Addgene plasmid # 220751 ; http://n2t.net/addgene:220751 ; RRID:Addgene_220751) -
For your References section:
Analyzing mRNA-protein complexes using a yeast three-hybrid system. Bernstein DS, Buter N, Stumpf C, Wickens M. Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4. 10.1016/S1046-2023(02)00015-4 PubMed 12054889
