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AAVS1 SA-2A-puro-pA donor
(Plasmid #22075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 5890
  • Vector type
    ZFN donor
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SA-Puro
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    712

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains a non-functional ccdB gene. The use of ccdB survival cells may be needed when subcloning or modifying this vector if the number of transformants is low.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1 SA-2A-puro-pA donor was a gift from Rudolf Jaenisch (Addgene plasmid # 22075 ; http://n2t.net/addgene:22075 ; RRID:Addgene_22075)

  • For your References section:

    Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Nat Biotechnol. 2009 Aug 13. ():. 10.1038/nbt.1562 PubMed 19680244
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