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Addgene

pAAV-CAG-NIR-FbLAG16
(Plasmid #220749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-mRuby2
  • Backbone manufacturer
    Addgene #99123
  • Backbone size w/o insert (bp) 5171
  • Vector type
    AAV, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NIR-FbLAG16
  • Species
    Synthetic
  • Insert Size (bp)
    858
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank: MW627294.1 (part of the insert). To make pAAV-CAG-NIR-FbLAG16, NIR-FbLAG16 was amplified and swapped with mRuby2 in pAAV-CAG-mRuby2 (Addgene #99123) using KpnI/EcoRI restriction enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-NIR-FbLAG16 was a gift from Vladislav Verkhusha (Addgene plasmid # 220749 ; http://n2t.net/addgene:220749 ; RRID:Addgene_220749)

  • For your References section:

    Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation. Barykina N et al.. Nat Commun 15, 7788 (2024) 10.1038/s41467-024-51857-x
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