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pET-NIR-FbLAG30
(Plasmid #220748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5410
  • Vector type
    Bacterial Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NIR-FbLAG30
  • Species
    Synthetic
  • Insert Size (bp)
    858
  • Mutation
    For bacterial cytoplasmic expression, the pelB signaling sequence (AAATACCTG...ATGGCCATG) was deleted
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank: MW627294.1 (part of the insert). To make pET-NIR-FbLAG30, NIR-FbLAG30 was amplified and inserted to pET22b(+) using NdeI/BamHI restriction enzymes. For cytoplasmic expression pelB signaling sequence (AAATACCTG...ATGGCCATG) was deleted by PCR with a forward primer.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-NIR-FbLAG30 was a gift from Vladislav Verkhusha (Addgene plasmid # 220748 ; http://n2t.net/addgene:220748 ; RRID:Addgene_220748)

  • For your References section:

    Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation. Barykina N et al.. Nat Commun 15, 7788 (2024) 10.1038/s41467-024-51857-x
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