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Addgene

pGP-mTagBFP2
(Plasmid #220747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pG12
  • Backbone manufacturer
    GeneScript
  • Backbone size w/o insert (bp) 4110
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTagBFP2
  • Species
    Synthetic
  • Insert Size (bp)
    696
  • Promoter 12xUAS with TATA-box minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AMP-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make pG12-mTagBFP2, mTagBFP2 was amplified and swapped with SEAP in pG12-SEAP (Addgene #170271) using BamHI/NotI restriction enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-mTagBFP2 was a gift from Vladislav Verkhusha (Addgene plasmid # 220747 ; http://n2t.net/addgene:220747 ; RRID:Addgene_220747)

  • For your References section:

    Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation. Barykina N et al.. Nat Commun 15, 7788 (2024) 10.1038/s41467-024-51857-x
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