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Addgene

pNLS-VP16-NIR-Fb(718)
(Plasmid #220745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIR-FbGFPY58/C-JIP
  • Backbone manufacturer
    Addgene #184684
  • Backbone size w/o insert (bp) 5006
  • Vector type
    Mammalian Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIR-Fb(718)
  • Species
    Synthetic
  • Insert Size (bp)
    1290
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS-VP16 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank: MW627296.1 (part of the insert). To make pNLS-VP16-NIR-Fb(718), VP16 was amplified from a pQP-T2A plasmid (Addgene #102583) and inserted into a pcDNA3.1 plasmid, containing the 15 aa-long linker (Gly3SerGly4SerGly3SerGly2) (Addgene #184684) using KpnI/BamHI restriction enzymes, and NIR-Fb(718) was inserted using NotI/XbaI restriction enzymes. A nuclear localization signal (NLS) was added to VP16-NIR-Fb(718) fusion by PCR amplification.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNLS-VP16-NIR-Fb(718) was a gift from Vladislav Verkhusha (Addgene plasmid # 220745 ; http://n2t.net/addgene:220745 ; RRID:Addgene_220745)

  • For your References section:

    Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation. Barykina N et al.. Nat Commun 15, 7788 (2024) 10.1038/s41467-024-51857-x
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