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Addgene

pNIR-Fb(718)
(Plasmid #220742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5045
  • Vector type
    Mammalian Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIR-Fb(718)
  • Species
    Synthetic
  • Insert Size (bp)
    825
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank: MW627296.1 (part of the insert). To make pNIR-Fb(718), GBP1 nanobody was amplified from pcDNA3.1-NSlmb-vhhGFP4 plasmid (Addgene #35579) and then internally fused to miRFP718nano amplified from pcDNA-miRFP718nano (Addgene #195744). Construct was inserted to pcDNA3.1 plasmid using KpnI and EcoRI restriction enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIR-Fb(718) was a gift from Vladislav Verkhusha (Addgene plasmid # 220742 ; http://n2t.net/addgene:220742 ; RRID:Addgene_220742)

  • For your References section:

    Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation. Barykina N et al.. Nat Commun 15, 7788 (2024) 10.1038/s41467-024-51857-x
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