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Addgene

pEB354
(Plasmid #22069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKT25
  • Backbone size (bp) 3453
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    pT25link
  • Tag / Fusion Protein
    • T25 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTGACCAGCGGCGATTCGGTGACCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB354 was a gift from Emmanuelle Bouveret (Addgene plasmid # 22069 ; http://n2t.net/addgene:22069 ; RRID:Addgene_22069)

  • For your References section:

    A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. Gully D, Bouveret E. Proteomics. 2006 Jan . 6(1):282-93. 10.1002/pmic.200500115 PubMed 16294310
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