pIII/MS2-2
(Plasmid
#220630)
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Purpose(Empty Backbone) For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescriptIIKS+
- Backbone size (bp) 7887
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene QC found an indel between SK primer and RPR1 promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIII/MS2-2 was a gift from Marvin Wickens (Addgene plasmid # 220630 ; http://n2t.net/addgene:220630 ; RRID:Addgene_220630) -
For your References section:
Analyzing mRNA-protein complexes using a yeast three-hybrid system. Bernstein DS, Buter N, Stumpf C, Wickens M. Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4. 10.1016/S1046-2023(02)00015-4 PubMed 12054889
