pMS2-1
(Plasmid
#220627)
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Purpose(Empty Backbone) Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescriptII KS+
- Backbone size (bp) 4545
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMS2-1 was a gift from Marvin Wickens (Addgene plasmid # 220627 ; http://n2t.net/addgene:220627 ; RRID:Addgene_220627) -
For your References section:
Analyzing mRNA-protein complexes using a yeast three-hybrid system. Bernstein DS, Buter N, Stumpf C, Wickens M. Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4. 10.1016/S1046-2023(02)00015-4 PubMed 12054889
