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Addgene

Perturb-FISH
(Plasmid #220626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiGuide-puro
  • Backbone manufacturer
    Feng Zhang Lab
  • Backbone size w/o insert (bp) 8326
  • Total vector size (bp) 10207
  • Modifications to backbone
    gRNA is placed under a U6T7 promoter, and uses an optimized scaffold sequence
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA
  • gRNA/shRNA sequence
    filler needs to be replaced with appropriate guide library
  • Species
    Synthetic
  • Promoter BsmBI

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    After design, the plasmid was assembled by Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.30.569494 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Perturb-FISH was a gift from Samouil Farhi (Addgene plasmid # 220626 ; http://n2t.net/addgene:220626 ; RRID:Addgene_220626)

  • For your References section:

    Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits. Binan L, Jiang A, Danquah SA, Valakh V, Simonton B, Bezney J, Manguso RT, Yates KB, Nehme R, Cleary B, Farhi SL. Cell. 2025 Mar 10:S0092-8674(25)00197-7. doi: 10.1016/j.cell.2025.02.012. 10.1016/j.cell.2025.02.012 PubMed 40081369
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