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Addgene

pTEF1_MS2
(Plasmid #220625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Backbone size (bp) 8291
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21-DE3 can be used for protein production.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTEF1_MS2 was a gift from Marvin Wickens (Addgene plasmid # 220625 ; http://n2t.net/addgene:220625 ; RRID:Addgene_220625)

  • For your References section:

    Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase. Preston MA, Porter DF, Chen F, Buter N, Lapointe CP, Keles S, Kimble J, Wickens M. Nat Methods. 2019 May;16(5):437-445. doi: 10.1038/s41592-019-0370-6. Epub 2019 Apr 15. 10.1038/s41592-019-0370-6 PubMed 30988468
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