MAP72_MS2
(Plasmid
#220623)
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Purpose(Empty Backbone) Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLeu2 YEplac181
- Backbone size (bp) 5000
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Vector typeYeast Expression ; shuttle vector
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAP72_MS2 was a gift from Marvin Wickens (Addgene plasmid # 220623 ; http://n2t.net/addgene:220623 ; RRID:Addgene_220623) -
For your References section:
Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase. Preston MA, Porter DF, Chen F, Buter N, Lapointe CP, Keles S, Kimble J, Wickens M. Nat Methods. 2019 May;16(5):437-445. doi: 10.1038/s41592-019-0370-6. Epub 2019 Apr 15. 10.1038/s41592-019-0370-6 PubMed 30988468