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Addgene

Leu2 2u
(Plasmid #220622)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220622 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 5738
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    empty vector
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    LEU2 (a.k.a. YCL018W)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Leu2 2u was a gift from Marvin Wickens (Addgene plasmid # 220622 ; http://n2t.net/addgene:220622 ; RRID:Addgene_220622)
  • For your References section:

    Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase. Preston MA, Porter DF, Chen F, Buter N, Lapointe CP, Keles S, Kimble J, Wickens M. Nat Methods. 2019 May;16(5):437-445. doi: 10.1038/s41592-019-0370-6. Epub 2019 Apr 15. 10.1038/s41592-019-0370-6 PubMed 30988468