Leu2 2u
(Plasmid
#220622)
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Purposeempty backbone for shuttle vector construction
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Total vector size (bp) 5738
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Leu2 2u was a gift from Marvin Wickens (Addgene plasmid # 220622 ; http://n2t.net/addgene:220622 ; RRID:Addgene_220622) -
For your References section:
Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase. Preston MA, Porter DF, Chen F, Buter N, Lapointe CP, Keles S, Kimble J, Wickens M. Nat Methods. 2019 May;16(5):437-445. doi: 10.1038/s41592-019-0370-6. Epub 2019 Apr 15. 10.1038/s41592-019-0370-6 PubMed 30988468