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Addgene

gCBEv3
(Plasmid #220618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
  • Alt name
    nSpCas9(2-1047;D10A)-XTEN-hUNG2(del1-88;N213D,K184A,A214V)-XTEN-Cas9(1064-1368)
  • Species
    Synthetic
  • Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
  • Promoter hU6, EF1a

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.01.573809 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gCBEv3 was a gift from Huawei Tong (Addgene plasmid # 220618 ; http://n2t.net/addgene:220618 ; RRID:Addgene_220618)

  • For your References section:

    Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase. Tong H, Wang H, Wang X, Liu N, Li G, Wu D, Li Y, Jin M, Li H, Wei Y, Li T, Yuan Y, Shi L, Yao X, Zhou Y, Yang H. Nat Commun. 2024 Jun 8;15(1):4897. doi: 10.1038/s41467-024-49343-5. 10.1038/s41467-024-49343-5 PubMed 38851742
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