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pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
(Plasmid #220613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-FlpO
  • Backbone manufacturer
    Karl Deisseroth
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
  • Species
    Synthetic
  • Promoter EF1a
  • Tag / Fusion Protein
    • FLAG; ArgiNLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ArgiNLS system was conceptualized and developed by Eric Szelenyi.

Please visit https://pubmed.ncbi.nlm.nih.gov/38045271/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1 was a gift from Sam Golden (Addgene plasmid # 220613 ; http://n2t.net/addgene:220613 ; RRID:Addgene_220613)

  • For your References section:

    An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single cells. Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B, Zweifel LS, Golden SA. Proc Natl Acad Sci U S A. 2024 Aug 6;121(32):e2320250121. doi: 10.1073/pnas.2320250121. Epub 2024 Jul 29. 10.1073/pnas.2320250121 PubMed 39074275
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