pAAV-CAG-FLEX-ArgiNLS-EGFP
(Plasmid
#220601)
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PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV-FLEX
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Backbone manufacturerScott Sternson
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesOther
- Promoter CAG
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Tag
/ Fusion Protein
- ArgiNLS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://pubmed.ncbi.nlm.nih.gov/38045271/ for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAG-FLEX-ArgiNLS-EGFP was a gift from Sam Golden (Addgene plasmid # 220601 ; http://n2t.net/addgene:220601 ; RRID:Addgene_220601) -
For your References section:
An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single-cells. Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B, Zweifel LS, Golden SA. bioRxiv [Preprint]. 2023 Dec 12:2023.11.22.568319. doi: 10.1101/2023.11.22.568319. 10.1101/2023.11.22.568319 PubMed 38045271