pBS-MCS1M(1)Kana-mCMV-TK-pA
(Plasmid #22060)

• Depositing Lab: Maria Castro
• Publication: King et al J Virol. 2008 May . 82(9):4680-4.

Backbone

• Vector backbone: pBS-MCS1(8)-Kana
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TK
• Alt name: thymidine kinase
• Insert Size (bp): 1400

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: the TK sequence is not listed in the full plasmid sequence, although it was detected in Addgene's sequencing results.