pAAV-EF1a-ArgiNLS-mGreenLantern
(Plasmid
#220596)
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PurposeConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-EF1a-FlpO
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Backbone manufacturerKarl Deisseroth
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGreenLantern
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SpeciesOther
- Promoter EF1a
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Tag
/ Fusion Protein
- ArgiNLS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The ArgiNLS system was conceptualized and developed by Eric Szelenyi.
Please visit https://pubmed.ncbi.nlm.nih.gov/38045271/ for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-ArgiNLS-mGreenLantern was a gift from Sam Golden (Addgene plasmid # 220596 ; http://n2t.net/addgene:220596 ; RRID:Addgene_220596) -
For your References section:
An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single cells. Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B, Zweifel LS, Golden SA. Proc Natl Acad Sci U S A. 2024 Aug 6;121(32):e2320250121. doi: 10.1073/pnas.2320250121. Epub 2024 Jul 29. 10.1073/pnas.2320250121 PubMed 39074275