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Addgene

MigR1 GFP Mouse PHGDH
(Plasmid #220591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MigR1 GFP
  • Backbone manufacturer
    Warren Pear (Addgene plasmid # 27490)
  • Vector type
    Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ms PHGDH
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1871
  • Entrez Gene
    Phgdh (a.k.a. 3-PGDH, 3PGDH, 4930479N23, A10, PGAD, PGD, PGDH, SERA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (unknown if destroyed)
  • 3′ cloning site HpaI (unknown if destroyed)
  • 5′ sequencing primer GTC GTT AAC CCACC ATGGCCTTCGCAAATCTGCGCAAAG
  • 3′ sequencing primer gac gtt aac tca gaa gca gaa ctg gaa agc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MigR1 GFP Mouse PHGDH was a gift from Russell Jones (Addgene plasmid # 220591 ; http://n2t.net/addgene:220591 ; RRID:Addgene_220591)
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