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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSTK120
- Backbone size w/o insert (bp) 28750
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructions32C and 160 RPM
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCMV-TK-pA expression cassette
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Alt nameHSV1-TK
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Alt namethymidine kinase
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Alt nameTK
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SpeciesHerpes Simplex Type 1
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Insert Size (bp)3862
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EagI (not destroyed)
- 3′ cloning site EagI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSTK120-mCMV-TK was a gift from Maria Castro (Addgene plasmid # 22054 ; http://n2t.net/addgene:22054 ; RRID:Addgene_22054) -
For your References section:
High-capacity adenovirus vector-mediated anti-glioma gene therapy in the presence of systemic antiadenovirus immunity. King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, Palmer D, Ng P, Lowenstein PR, Castro MG. J Virol. 2008 May . 82(9):4680-4. 10.1128/JVI.00232-08 PubMed 18287240
Map uploaded by the depositor.
